Re: [Histonet] F4/80 quick question
Yes, and it works very well. It is in our panel of murine antibodies. Our
frozen sections are air dried overnight then fixed in acetone 75%/ absolute
ethanol 25% 5 min at RT, then directly into buffer after fixation without
any further air drying.
We did a dilution panel for our laboratory conditions, and used a goat
antiRat F(ab')2 secondary, Strepavidin-HRP/AEC with all the appropriate
blocking step.
We use this antibody for both immunohistochemistry and immunofluorescence
staining.
At 09:47 AM 12/4/2006, you wrote:
>Does F4/80 from Serotec work on frozen sections of mouse tissue?
>--
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)
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