Re: [Histonet] Decreased Staining on Controls

From:Rene J Buesa

  Control sections oxidize and the antigenic activity decreases (I am sending you under separate cover a paper I wrote on the subject).
  I always tried to have enough controls for 1 week's worth of IHC procedures. After 2-3 weeks the weakening started.
  Even at low temperature this will occur (although more slowly because of the reduction in the oxidation speed because of a lower temperature).
  A student I tutored for a BMS degree (in the UK) wrote his thesis on this subject. The best way she found in protecting the sections was storing them in a nitrogen atmosphere, but this, as a normal practice, will be somewhat difficult.
  The safest way is to have just what you need. Several months is too much.
  Hope this (and the article) will help you!
  René J.

Annette Hall  wrote:

We are having problems with are CD15 and CD30 antibodies using the
DakoCytomation Artisan. The controls seem lighter than when we first started
with this instrument 4 years ago. Our instruction has been to use "fresh"
controls. We cut controls and store them at 4 deg C for a few months. In
your experience, what do you consider fresh? How long do you keep cut slides
controls before you toss them?

Annette J Hall, MT
Micro/Cyto/Histo Supervisor
United Clinical Labs
205 Bluff Street
Dubuque, IA 52001
Phone: 563.556.2010 x131

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