RE: [Histonet] Eosin Y aqueous

From:"Kemlo Rogerson"

Has anyone used Aqueous Eosin Y from Sigma for H&E staining frozen
sections. It always seems to leach out of the tissue - I have tried
staining for between 2-10minutes (the staining is always very strong
before washing). I have tried mounting in aqueous mountant (Vector)
after washing with tap water (5min) and also in DPX after dehydrating in
alcohol and clearing in zylene. In both instances most of the Eosin
staining was washed away or leached out while the mountant was setting. 
I will buy some different eosin if I cant get this one to work but just
wondered if anyone has encountered similar problems.

Tried using calcium ions as an accentuator?

Kemlo Rogerson
Pathology Manager
Ext  3311
DD   01934 647057
Mob 07749 754194
Pager 07659 597107
Once you are in the orbit of your destiny, weightlessness is the only
result. --Baba Amte 

This e-mail is confidential and privileged. If you are not the intended
recipient please accept my apologies; please do not disclose, copy or
distribute information in this e-mail or take any action in reliance on
its contents: to do so is strictly prohibited and may be unlawful.
Please inform me that this message has gone astray before deleting it.
Thank you for your co-operation

Histonet mailing list

<< Previous Message | Next Message >>