[Histonet] Histonet] Tube cross sections in Frozens
From: | "Stephen Peters M.D." |
Dear Renee,
This can be accomplished easily using frozen block cryoenmbedding. This is a two step
embedding process. First the tissue is frozen in a block of embedding medium. This now firm easy to cut frozen block is then sectioned on edge. The resulting slices are then embedded on edge either face down in a well ( mold) or just by lying it face up on a chuck. You can do the first freeze between any two pieces of freezing temp metal. This process is detailed at:
http://pathologyinnovations.com/frozen_block_embedding.htm
Stephen
Stephen
Stephen Peters M.D.
Vice Chairman of Pathology
Hackensack University Medical Center
201 996 4836
Pathology Innovations, LLC
410 Old Mill Lane,
Wyckoff, NJ 07481
201 847 7600
www.pathologyinnovations.com
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