[Histonet] Histonet] Tube cross sections in Frozens

From:"Stephen Peters M.D."

Dear Renee,
   
  This can be accomplished easily using frozen block cryoenmbedding. This is a two step 
  embedding process. First the tissue is frozen in a block of embedding medium. This now firm easy to cut frozen block is then sectioned on edge. The resulting slices are then embedded on edge  either face down in a well  ( mold) or just by lying it face up on a chuck. You can do the first freeze between any two pieces of freezing temp metal. This process is detailed at: 
   
  http://pathologyinnovations.com/frozen_block_embedding.htm
   
  Stephen
   
   
  Stephen
   


Stephen Peters M.D. 
Vice Chairman of Pathology
Hackensack University Medical Center 
201 996 4836
 
Pathology Innovations, LLC 
410 Old Mill Lane, 
Wyckoff, NJ 07481 
201 847 7600 
www.pathologyinnovations.com 




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