Re: [Histonet] preservation of unstained 20 um sections on slides for later DNA research

From:"Helen Fedor"

We have had pretty good success with drying them overnight, vertically, then  putting them face to face, wrapping them tightly in parafilm and then putting them into a ziploc and expelling the air.
And then store at -20.
We have only done this on 4 to 5 micron sections, and have them stored for up to 3 years and they retain their antigenicity fairly well.
Helen

Helen L. Fedor B.S.
Johns Hopkins University
Pathology Department
600 N Wolfe St
Marburg Room 406
Baltimore MD 21287
email: hfedor@jhmi.edu
Phone: 410 614-1660
Pager: 410 283-3419

WARNING: E-mail sent over the Internet is not secure. Information sent by e-mail may not remain confidential. 
DISCLAIMER: This e-mail is intended only for the individual to whom it is addressed. It may be used only in accordance with applicable laws. If you received this e-mail by mistake, notify the sender and destroy the e-mail.



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


<< Previous Message | Next Message >>