Re: [Histonet] Mouse liver tissue processing

From:"Andrea T. Hooper"

Hi Luis!

Are these livers being processed with a human processing protocol? If 
so you need to ask the facility to use an abbreviated protocol 
especially for mouse tissue. Do you know the details of the 
processing protocol they have been using?

I find mouse livers are notoriously "crunchy" especially when 
overprocessed. Even when using the special mouse tissue processing 
protocol I still sometimes find the need to do a nice icy water bath 
soak for a good 10-20 minutes on some blocks to get good sections.

Good luck!

>Hi everyone
>We are having a real serious problem getting good sections from mouse
>livers.  The tissue curls and shreds out of the block as soon as it comes in
>contact with the knife, end up with a section that has the exact outline of
>the tissue but no tissue. The tissue consistency could best be described as
>"dry?" Interestingly, it is only the normal livers that have this problem.
>We have tried sectioning thick, thin, no soak, cold soak......but out of
>ideas. Anyone have any suggestions? Unfortunately we have no control over
>the tissue processing, but perhaps can convince to change.
>Thanks Luis
>Happy Holidays to all!!
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