Re: [Histonet] ER quantitative controls

From:Rene J Buesa

Hello all:
  My mistake! I was referring to the Her-2-Neu test, and Barb's question was about ER!
  Regarding ER we never reported it as positive grades; just as + or - Our pathologists considered it irrelevant due to many variables that could affect the + degree of the reaction.
  My excuses to Barb and all!
  René J. wrote:

Hello all -

For those labs reporting ER results as quantitative (1+, 2+, 3+) --- two

1) Does your control tissue show representation of the 3 different grades
- meaning that there is tissue staining as 1+, another piece staining as
2+, and a third piece showing 3+ staining? Or do you simply show a
positive reaction?

2) If you do show representation of 1+, 2+, 3+ could you please share your
process of obtaining control tissue?

Thank you for any comments!

Barb Moe
Gundersen Lutheran Medical Center
La Crosse WI

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