Re: [Histonet] ER quantitative controls

From:Rene J Buesa

Hello Barb:
  We used the Dako HercepTest and there are positive controls with it (they are paraffin embedded cultured cells pearls). Since there are only few slides/test kit, we used to 
  batch our tests and use 1 control slide/run of several cases.
  Sometimes we had + cases to be used as controls but a case has a particular
  positivity condition, and the slides provided by Dako contain the different + values in the same slide.
  Perhaps you could contact them and find out if they can sell you + slides.
  René J. wrote:

Hello all -

For those labs reporting ER results as quantitative (1+, 2+, 3+) --- two

1) Does your control tissue show representation of the 3 different grades
- meaning that there is tissue staining as 1+, another piece staining as
2+, and a third piece showing 3+ staining? Or do you simply show a
positive reaction?

2) If you do show representation of 1+, 2+, 3+ could you please share your
process of obtaining control tissue?

Thank you for any comments!

Barb Moe
Gundersen Lutheran Medical Center
La Crosse WI

Histonet mailing list

Do You Yahoo!?
Tired of spam?  Yahoo! Mail has the best spam protection around 
Histonet mailing list

<< Previous Message | Next Message >>