RE: [Histonet] sealing slides with diluted permount

From:"Malcolm McCallum"

The research I do has involved simply mounting specimens on slides with permount.  With reference to sealing, I would appreciate any comments on this technique.  What are some methods and how does this help with longivity of the slide?
Malcolm L. McCallum
Assistant Professor
Department of Biological Sciences
Texas A&M University Texarkana
2600 Robison Rd.
Texarkana, TX 75501
O: 1-903-233-3134
H: 1-903-791-3843


From: on behalf of Caroline Bass
Sent: Mon 12/19/2005 12:51 PM
To: Histonet (E-mail)
Subject: [Histonet] sealing slides with diluted permount


I have some slides of GFP expressing tissue.  I am using the prolong 
gold mounting media from Molecular Probes to mount the coverslips but 
now I have to seal them.  I have read that the best way to do this is 
with diluted permount.  Unfortunately I have never sealed slides 
before and am having a hard time visualizing the process.

What is a good starting dilution for the permount?  I was thinking 
1:10 with xylenes to start, but this is just a guess.  How exactly do 
you apply the permount?  Can you just pipet it around the edges or do 
you need to brush it on in some manner?  Does it have to be flush 
with the coverslip or can you have some buildup?

Any advice would be appreciated.


Caroline Bass

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