[Histonet] Quantum dot immunohistochemistry


Dear Histonet users,

I would like to ask you for advice. I am starting with quantum dot
conjugated secondary antibody immunohistochemistry. I have tried one trial
with the same protocol I use with Alexa Fluor. Secondary antibody
concentration 1:100 (1:50 is recommended by chemicon), but I have terrible
background. Does anybody have idea what is "best" working dilution?


Thank you very much

Happy New Year

Jakub Otahal


 Jakub Otahal MD,PhD
Department of Developemental Epileptology 
Institute of Physiology 
Czech Academy of Sciences 
Videnska 1083 
142 20 Prague 4 
Czech Republic 
tel: +420 241062495



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