[Histonet] Q: Drop of Ammonia to Avoid Wrinkling
|From:||Lawrence D Lanberg/O/VCU |
A Happy Holiday Season to All!
When I = joined the listserv a week ago, I saw an interesting
(archived)&n= bsp;tip on using ammonia to avoid wrinkling on semi-thin
plastic = sections. With my P.I.s approval, I've purchased a bottle of
10% ammonia wa= ter to try this. I don't know if anyone receiving this
message is familiar = with it, but I was hoping someone might be to
able clarify my understanding= , beforehand:
After I release the sections onto&= nbsp;the tiny pool of water on my
slide, I add one small drop of ammon= ia and then dry and stain as
usual. That's the way I interpreted what I rea= d. Does this sound
ps. I make 990 nm sections of murine retina, the eye is embedd= ed in
Spurr's Formula and I use toluidene blue stain.
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