[Histonet] Mouse Autoradiography of fixed frozen tissue
I was hoping that I can get some assistance on a
project I'm going to do. An Investigator here would like to look at
radiolabeled mouse Sm. intestines for there specific radiolabeled protein.
My problem is that I don't want to use our only processor to process
radioactive material ( I 125 or H3, not sure yet) My other histology
colleges may not like me so much if I did that.
So I plan on fixing the material in 10% NBF (24-48 hours), washing
in water (Blot) then cryopreserving them (LN2 Isopentane). This way I can
Cryosection and decontaminate the less used cryostat. Next I wash in
water and lastly dipping slides in Photographic Emulsion and wait, and
wait....My question is, has anyone done this that could help me out with
the finer details and will this be OK? I've done this with paraffin
samples dip wait 2,4,7,14, 21, etc days for a signal, but not with
If anyone has done this and could help me out I'd really appreciate it..
Sr. Research Associate
Abbott Bioresearch Center
100 Research Drive
Worcester, MA 01605-4341
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