[Histonet] FW: artifact

From:"Santana, Diane"

>  -----Original Message-----
> From: 	Santana, Diane  
> Sent:	Tuesday, December 20, 2005 10:20 AM
> To:	'histonet@lists.utsouthwestern.edu'
> Subject:	artifact
> I am having a problem with artifact on some of my GI bx's. Actually a
> paper published by Anatech "The Innovator", summer2004 shows this same
> artifact on the front page. They believe it is a fixation problem, and or
> retrieval by the endo Drs. My pathologist rules both these ideas out. I
> tend to agree with him, at lease on the fixation. Our bx's go into the
> processor around 5 pm and the bx run starts around 2 am. 
> It is a loss of nuclear detail. This artifact can be on a small section of
> a bx. Even if there is 5 bx's in one cassette maybe 1 will have this
> artifact where the others look great. I thought at first it was a clearing
> problem or nuclear bubbling I changed times in clear rite and oven. It did
> not eliminate the problem. My pathologist has seen this by other labs
> also, but very seldom. For us it could be 1 or 2 slides a day. Sometimes
> not at all. Weekend or weekdays. No consistently. The best way I can
> describe it is that it looks like a smudge.
> Our bx run is this;
> 10% NBF 30 min ea x2
> 80% Alcohol 10mins 
> 95% alcohol 10 mins ea x2
> 100% alcohol 17 mins ea x3
> Clear rite 22 mins ea x2
> Paraffin 15 mins ea x3
>  I would appreciate any feedback on this.
> Diane Santana
> Pentucket Medical Assoc.
> Haverhill, Mass.

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