[Histonet] Dako streptavidin / FITC for immuno's

From:"PALMER Jason \(SVHM\)"

Dear all,

Just wondering if anybody has used Dako streptavidin / FITC (F0422) in an immunohisto or immunocytochemistry run, and roughly what dilution you found to be best? We have very limited material to work with and so can't really do a titration to find the best dilution for ourselves (which is what we would normally do). It is to be used with a Stro-1 primary and biotinylated goat anti mouse secondary on cultured cells. The spec sheet has a suggested dilution range for flow cytometry only. Much obliged if anyone can help.



Jason Palmer
Bernard O'Brien Institute of Microsurgery

42 Fitzroy St, Fitzroy Victoria 3065
tel +61 3 9288 4018
fax +61 3 9416 0926
email: palmerj@svhm.org.au

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