FW: [Histonet] pancreas special stains

From:"Bharat Mani"



Bharat Mani
Email: bharatm@glenmarkpharma.com


-----Original Message-----
From: Bharat Mani 
Sent: Thursday, December 22, 2005 9:57 AM
To: 'Rene J Buesa'; 'histonet-bounces@lists.utsouthwestern.edu '; 'sjchtascp@yahoo.com'
Subject: RE: [Histonet] pancreas special stains

Dear Rene & Steve

We have tried the following technique - http://home.primus.com.au/royellis/ST/PANCREAS.htm and observed satisfactory results. 

Rene, would be glad if you could share the procedures you have tried...

Thanks & Regards

Bharat Mani
Email: bharatm@glenmarkpharma.com

 
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Thursday, December 22, 2005 2:37 AM
To: Steven Coakley; Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] pancreas special stains

Steve:
  I strongly recommend you to use the Mallory-Aaan classical trichromic stain widely
  used by Alexander Alexandrovich Maximow, it will differentiate α, =DF and delta cells.
  Also Bowie's procedure will stain α cells blue; ▀ cells purple and delta cells red.
  I have used both procedures with very good results (with pancreas fixed in Zenker's though!).
  If you don't have the procedure, please let me know.
  RenÚ J.
Steven Coakley  wrote:
  Has anyone had any experiance with any of the special stains for pancreatic alpha, beta and delta cells.

Steve

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