Re: [Histonet] fixation in 70% alcohol

From:Bruce Abaloz

Hi fawn,

you will no doubt receive replies that 70%ETOH is not a 
fixative....we have been using it for over a decade now & it does a 
beautiful job. I discussed this @ a workshop in Toronto last year 
with Histology colleagues/Workshop on 'Fixation'. After initial 
'gasps' (how could you use that) that it was my preference/choice of 
fixatives & gave us excellent results, another seven Histologists in 
the workshop 'admitted that they also use it in their labs......of 
course we still use 10% NBF, Bouins etc. when asked to fix in them 
specifically, but 90% of our tissues are fixed in 70% ETOH .......as 
this is a Research Lab. we have time on our side - minimum time for 
skin lesions etc. 24hrs. but today we also have the luxury of 
infiltrating under vacuum....
Cheers,

Bruce in OZ
-- 
  BRUCE ABALOZ 
PH:61383446282
  HISTOLOGIST 
FAX:61383447909
  DEPT.of ZOOLOGY 
EMAIL: brucea@unimelb.edu.au
  THE UNIVERSITY Of MELBOURNE.  VICTORIA. AUSTRALIA 3010
     Nobody Can Make You Feel Inferior Without YOUR Permission - 
Eleanor Roosevelt
                       DANCE LIKE NO-ONE'S WATCHING
                 Q: What's a specimen?                 A: An Italian 
astronaut. 
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