Re: [Histonet] Specail stains Negative controls

From:"Bryan Hewlett"

Debby and Jeff,

Negative control slides are useful for GMS, Gram and AFB in order to 
eliminate false positives due to contamination from the reagents, washing, 
carry-over etc.



----- Original Message ----- 
From: "Smith, Jeffery D. (HSC)" 
To: "Grant, Debra, R" ; 
Sent: Friday, December 02, 2005 2:23 PM
Subject: RE: [Histonet] Specail stains Negative controls

   Routine special stains only require a positive control as you are looking 
to see if the stain worked or not.  Specimen is either positive or negative. 
No need for negative control.  IHC uses both pos. and neg. controls because 
you are looking for varying intensities of staining as well as pos. or neg.

Best of Luck,


Hi Histonetters,

Does anyone run negative control slides with their GMS, AFB, and Gram
special stains, and if so why?

Kind Regards,

Debby R. Grant HT(ASCP)
Histology Coordinator
The Children's Mercy Hospital & Clinics
2401 Gillham Rd.
Kansas City, Missouri 64108
lab (816)234-3827
fax (816) 802-1492

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