Re: [Histonet] RE: Paraffin sections

From:Maria Mejia

This type of experimenting would be interesting to check out especially 
lower percentages of BSA. Andrea, I would agree that this overnight storage
step probably is antigen dependent (Chris, that's what I meant by too much)
and it would require a whole gamut testing of antibodies to see which 
positively and which would not...if one is inclined to do so.


Maria Bartola Mejia
Smith-Kettlewell Eye Research Institute
San Francisco, CA 94115
Phone: (415)-345-2185

Andrea T. Hooper wrote:

> Very interesting Chris! I have found sometimes this works and 
> sometimes it doesn't and you get complete loss of staining - 
> frustrating when one was just trying to save time to begin with!! I 
> imagine it's antigen dependent but I have not had time to check.
> In my opinion Betsy, if you can avoid it (because you are just doing 
> it to save time instead of setting up a workshop) then avoid it ... it 
> simply isn't worth the risk IMHO.
> At 9:52 AM +0100 12/2/05, C.M. van der Loos wrote:
>>    Betsy,
>>    For my NSH wet-workshops I tested the option of going up to endogenous
>>    PO blocking, HIER and then overnight storage at 4C in PBS with 1% BSA.
>>    Next  day  we  started at the protein blocking step and then the rest.
>>    Staining was as good as running the whole procedure in one day.
>>    Hope this info helps.
>>    Chris van der Loos, PhD
>>    Dept. of Pathology
>>    Academic Medical Center M2-230
>>    Meibergdreef 9
>>    NL-1105 AZ Amsterdam
>>    The Netherlands

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