Re: [Histonet] RE: Paraffin sections
This type of experimenting would be interesting to check out especially
lower percentages of BSA. Andrea, I would agree that this overnight storage
step probably is antigen dependent (Chris, that's what I meant by too much)
and it would require a whole gamut testing of antibodies to see which
positively and which would not...if one is inclined to do so.
Maria Bartola Mejia
Smith-Kettlewell Eye Research Institute
San Francisco, CA 94115
Andrea T. Hooper wrote:
> Very interesting Chris! I have found sometimes this works and
> sometimes it doesn't and you get complete loss of staining -
> frustrating when one was just trying to save time to begin with!! I
> imagine it's antigen dependent but I have not had time to check.
> In my opinion Betsy, if you can avoid it (because you are just doing
> it to save time instead of setting up a workshop) then avoid it ... it
> simply isn't worth the risk IMHO.
> At 9:52 AM +0100 12/2/05, C.M. van der Loos wrote:
>> For my NSH wet-workshops I tested the option of going up to endogenous
>> PO blocking, HIER and then overnight storage at 4C in PBS with 1% BSA.
>> Next day we started at the protein blocking step and then the rest.
>> Staining was as good as running the whole procedure in one day.
>> Hope this info helps.
>> Chris van der Loos, PhD
>> Dept. of Pathology
>> Academic Medical Center M2-230
>> Meibergdreef 9
>> NL-1105 AZ Amsterdam
>> The Netherlands
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