Re: [Histonet] Myelin in spinal cord

From:Rene J Buesa

Hi Gerald:
  Many years ago I used to work with lizard and frog spinal cords; I used paraffin embedding and there was not a problem.
  Besides the Luxol Fast Blue-Cresyl Etch Violet there are nomerous other well recognized techniques. I personally have used (and like) Lillie's and Olivecrona's method.
  I would advise you to change to paraffin embedding.
  Hope this will help you!
  René J.

""  wrote:


I have a problem and am in need of some advice.

I am working on spinal cord of zebrafish and will be staining for

myelin in fine detail. I have been strongly advised not to use paraffin embedding and so am currently doing gelatin embedded sectioning on a cryostat. Unfortunately this has led to tissue loss due to poor adherence to the slide. I tried superfrost plus and superfrost gold slides with no change. gelatin coating the slides

greatly improved this but staining clarity and specifity is very poor. I have tried varying times and even stains (Kultschitsky's verses Weil's) but no improvement.

Does anyone have any suggestions on how to improve this or what other other techniques (including possibly other stains) I could try?

Thanking you,

Gerald McKeon

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