We received the new lot of CD31 from Santa Cruz, and it is working, though we
are going to have to use it at a more concentrated diution than our current
(1:750).

We haven't had the time to see what the new concentration will be, but I'm
guessing somewhere around 1:200 or so.  If the CD31 you have is not working,
Santa Cruz will replace it to try it for yourself.  We got a couple of vials for
free as replacements.

Another alternative is CD34 (can give you some of the same information,
depedning on your work).  Abcam has a great rat polyclonal that works well in
FFPE mouse tissues.


Angela


Angela McNabola, MS, HT(ASCP)SLS, QIHC
Bayer Helathcare
400 Morgan Lane
West Haven, CT 06516
203-812-5001
angela.mcnabola.b@bayer.com



                                                                                                                                        
                      "Jackie M O'Connor"                                                                                               
                                To:       "Histonet (E-mail)"            
                      Sent by:                              cc:                                                                         
                      histonet-bounces@lists.utsouth        Subject:  [Histonet] FFPE CD31 on mouse                                     
                      western.edu                                                                                                       
                                                                                                                                        
                                                                                                                                        
                      12/13/2005 11:57 AM                                                                                               
                                                                                                                                        




I've been waiting for Santa Cruz to grow a new goat for the CD31 antibody
that USED TO work on FFPE murine tissue - but with no luck.
I've scoured the internet for two days looking for a viable replacement -
nada.
Does anyone have a method for murine CD31 in FFPE that works without the
Santa Cruz antibody?  I've tried the Pharmingen anti-mouse with AR - but
it didn't work.  Boo.

Jackie O'
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