Re: [Histonet] Cuting speed and sem-thin plastic sectioning

From:Lawrence D Lanberg/O/VCU

   Thanks  for  the  reply.  I make my own glass knives (part of the pro   tocol  for this lab) and I do attach a little boat on the back so that
   they  =  float  in diH20 as the ribbon is being made. I usually cut at
   990 nm.

   <= DIV>

   I also made a mistake in my initial post, I see. Its a= Sorvall MT6000
   - not 1000. As I said, when I cut on the higher speeds the = section -
   after  staining  and  under  400x mag - will appear as corrugated. St   riped. I am wonder if i try to go back to the 'soft' Spurr mix.


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