Re: [Histonet] Cuting speed and sem-thin plastic sectioning
From: | Lawrence D Lanberg/O/VCU |
Thanks for the reply. I make my own glass knives (part of the pro tocol for this lab) and I do attach a little boat on the back so that
they = float in diH20 as the ribbon is being made. I usually cut at
990 nm.
<= DIV>
I also made a mistake in my initial post, I see. Its a= Sorvall MT6000
- not 1000. As I said, when I cut on the higher speeds the = section -
after staining and under 400x mag - will appear as corrugated. St riped. I am wonder if i try to go back to the 'soft' Spurr mix.
LDL
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