We have recently changed how we deal with the negative control
situation.
We run one negative control slide per specimen block, using our most
aggressive pretreatment
protocol, per panel of antibodies.  We also document that within our
positive control tissue 
there are negative tissue elements.


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of patsy
ruegg
Sent: Friday, December 09, 2005 10:30 AM
To: 'Roxanne Soto'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] cap ihc info


This is an ongoing issue in IHC, when dealing with limited tissue how
can we afford to use up sections for negative controls on each block.  I
haven't been updated on this for awhile, just wondering what is
happening in the real world, are you including a negative (isotype
matched reagent in place of the primary antibody) for each block even
when it is a small biopsy specimen. Patsy 

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. #216
Aurora, CO 80010
720-859-4060
fax 720-859-4110
pruegg@ihctech.net
www.ihctech.net 
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roxanne
Soto
Sent: Friday, December 09, 2005 8:19 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] cap ihc info


       ______________________________________________________________

     >I  have  a  question  about  a particular IHC that I do on a daily
     basis. I do a PIN-4 cocktail
     >stain  on  prostate  cores. This cocktail contains p63, CK903, and
     P504S. My question is, do I
     >need to perform a negative control?
     >Right  now  we are not CAP certified, but we are looking to become
     certified. CLIA only
     >requires one negative per case, instead of one negative per block.
     We deal with very tiny
     >cores  and  I  am  currently following the CLIA guidelines in this
     matter. However, we are
     >looking  to  automate  and  work  on  CAP  accreditation  and this
     negative factor would greatly
     >increase  our volume and therefor would mean that we would need to
     get a stainer to
     >accommodate the volume.
     >I  know that whenever I do a CK903, I do not do a negative because
     the tissue has built in
     >controls. Is this now null and void because of the racemase?
     >Please advice.
     >Thank you
     >Roxanne Soto HT(ASCP)
     >Tampa
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