RE: [Histonet] cap ihc info
This is an ongoing issue in IHC, when dealing with limited tissue how can we
afford to use up sections for negative controls on each block. I haven't
been updated on this for awhile, just wondering what is happening in the
real world, are you including a negative (isotype matched reagent in place
of the primary antibody) for each block even when it is a small biopsy
specimen.
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. #216
Aurora, CO 80010
720-859-4060
fax 720-859-4110
pruegg@ihctech.net
www.ihctech.net
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roxanne Soto
Sent: Friday, December 09, 2005 8:19 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] cap ihc info
______________________________________________________________
>I have a question about a particular IHC that I do on a daily
basis. I do a PIN-4 cocktail
>stain on prostate cores. This cocktail contains p63, CK903, and
P504S. My question is, do I
>need to perform a negative control?
>Right now we are not CAP certified, but we are looking to become
certified. CLIA only
>requires one negative per case, instead of one negative per block.
We deal with very tiny
>cores and I am currently following the CLIA guidelines in this
matter. However, we are
>looking to automate and work on CAP accreditation and this
negative factor would greatly
>increase our volume and therefor would mean that we would need to
get a stainer to
>accommodate the volume.
>I know that whenever I do a CK903, I do not do a negative because
the tissue has built in
>controls. Is this now null and void because of the racemase?
>Please advice.
>Thank you
>Roxanne Soto HT(ASCP)
>Tampa
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