RE: [Histonet] Tissue Proccessing HELP!!!!!!!!!!!!!!

From:"Anne Van Binsbergen"

What kind of tissue?
Was it 'dried out' before placing in fixative?
What is your processing protocol
In my humble opinion - reprocessing this small bx will just make it

I have used VIP5's for the past 10 years and they are wonderful
processors. Its usually the times of the processing protocol that are
the 'devil' - OR (I have found this to be a HUUUGE factor - how long the
bx lies in 'fresh air' before it gets popped into formalin (beyond your
This term 'cooked' is loosely applied by Paths.

I have only one VIP and use the same programme overnight - for small
gastrics, mastectomies, lymph nodes, liver and renal cores - a whole
variety of tissue.
The 'latch' of the VIP cannot be 'loose' - the retort has a safety
mechanism - it wont process if it 'leaks' - perhaps the rubber gasket
was not snug in its housing (whoever last used/cleaned machine should

We often blame the equipment - when it is the operators fault
Cars are not to blame for accidents - drivers are!!!

-----Original Message-----
From: Jesus Ellin [] 
Sent: Wednesday, December 14, 2005 5:29 AM
Subject: [Histonet] Tissue Proccessing HELP!!!!!!!!!!!!!!

Hello everyone I have a real problem here, We have just accquired a
tissue proccesor (VIP 5) and found out the hard way like everything in
Histology, there is a problem with the proccessing.  Our pathologist are
claiming that the small biopsies are coming out cooked,  after reviewing
the slide myself with the pathologist it is not a cooked looked but
rather a glassy look and bubbly.  Later found out that the latch of the
tissue proccessor is not tight enough and is was allowing moisture in
and there was a lot of condensation.  I figured out what was wrong but
here is the kicker.

My Pathologist is wanting to take one of the small biopsies that was
proccessed and regress the tissue and then reproccess the small biopsy
by hand instead of throwing it back on the tissue proccessor.  The
biopsy is about .1 mm very tiny.  I need all the help that I can
get!!!!!!  So please fellow histo-netters share the knowledge with me.

Jesus Ellin
Yuma Regional Medical Center

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