RE: [Histonet] SDH protocol



Give this procedure a try. It worked very well for us when I worked at
Children's Hospital in Cincinnati. 
We purchased the substrate from SIGMA and used it, until it was gone.
The reaction should be a deep blue. Hope it works for you!


Beatrice DeBrosse-Serra
Allergan, Inc.
2525 Dupont Drive RD-2A
Irvine, CA 92612

-----Original Message-----
[] On Behalf Of Lewis,
Sent: Friday, December 09, 2005 7:19 AM
To: HISTONET (E-mail)
Subject: [Histonet] SDH protocol

Fellow Neuromuscular Labs!!
I am in need of a Great SDH protocol.  I have been struggling with this
stain for a long time.  I purchase new NBT and it works pretty well for
a few weeks.  Then is starts fading into nothing within 1month.   I use
a protocol of on the WashU website (St. Louis MO). It works better than
any other I have tried... but still very pale, However my pathologist
said that the SDH's in the past have always been more blue.  Mine come
out purple/ pink.  I have also used the protocol from past histologist
in this lab with no luck.  I must be missing some small technique that
isn't written in the protocol.  Please help!!!       

Sarah E. Lewis 
Childrens Research 
Center for Gene Therapy 
700 Childrens Dr Rm WA3112 
Columbus OH 43205 

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