RE: [Histonet] SDH protocol
Give this procedure a try. It worked very well for us when I worked at
Children's Hospital in Cincinnati.
We purchased the substrate from SIGMA and used it, until it was gone.
The reaction should be a deep blue. Hope it works for you!
2525 Dupont Drive RD-2A
Irvine, CA 92612
[mailto:email@example.com] On Behalf Of Lewis,
Sent: Friday, December 09, 2005 7:19 AM
To: HISTONET (E-mail)
Subject: [Histonet] SDH protocol
Fellow Neuromuscular Labs!!
I am in need of a Great SDH protocol. I have been struggling with this
stain for a long time. I purchase new NBT and it works pretty well for
a few weeks. Then is starts fading into nothing within 1month. I use
a protocol of on the WashU website (St. Louis MO). It works better than
any other I have tried... but still very pale, However my pathologist
said that the SDH's in the past have always been more blue. Mine come
out purple/ pink. I have also used the protocol from past histologist
in this lab with no luck. I must be missing some small technique that
isn't written in the protocol. Please help!!!
Sarah E. Lewis
Center for Gene Therapy
700 Childrens Dr Rm WA3112
Columbus OH 43205
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