RE: [Histonet] IHC on coverglass

From:"Sebree Linda A."

Only under extreme duress!  In our case, we mounted the coverslips on a
regular slide and stained it on our automated instrument (Ventana).  We
weren't very successful  but I think it was because these were bone
marrow smears that sat around air-dried in a file for who knows how
long.  We weren't informed that they had not been fixed in any manner
and we ended up losing most of the cells.  In a best case scenario it
might work a whole lot better.

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Clinical & Research Laboratory
DM223-VA
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX:  (608)262-7174



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Steven
Coakley
Sent: Monday, December 12, 2005 3:43 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC on coverglass


Does anyone out there do IHC on coverglass.  Appears to be a "tad"
tricky.
   
  Steve

			
---------------------------------
Yahoo! Shopping
 Find Great Deals on Holiday Gifts at Yahoo! Shopping 
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


<< Previous Message | Next Message >>