RE: [Histonet] FFPE CD31 on mouse

From:"Koelling, Ray"

Not too long ago I posted on Histonet our CD31 procedure we've used for a
couple years.  On mouse tissue using the Pharmingen antibody.  Works
beautifully but need to enzyme retrieve and amplify signal.  Should be in


Raymond Koelling
Pathology Research scientist
Amgen Corp.
Seattle, WA

-----Original Message-----
[]On Behalf Of Jackie M
Sent: Tuesday, December 13, 2005 8:57 AM
To: Histonet (E-mail)
Subject: [Histonet] FFPE CD31 on mouse

I've been waiting for Santa Cruz to grow a new goat for the CD31 antibody 
that USED TO work on FFPE murine tissue - but with no luck.
I've scoured the internet for two days looking for a viable replacement - 
Does anyone have a method for murine CD31 in FFPE that works without the 
Santa Cruz antibody?  I've tried the Pharmingen anti-mouse with AR - but 
it didn't work.  Boo. 

Jackie O'
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