RE: [Histonet] Anti-DIG peroxidase problem

From:"Tony Henwood"

Try using a FITC labelled probe, followed by a mouse or rabbit anti-FITC.
Then use your usual immunoperoxidase demonstration technique (ABC or polymer - I use a peroxidase-labelled polymer)


Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

-----Original Message-----
From: [] On Behalf Of Mikael Niku
Sent: Tuesday, 6 December 2005 6:09 PM
Subject: [Histonet] Anti-DIG peroxidase problem

Dear Histonetters,

I'm trying to set up tyramide amplification for my in situ 
hybridizations with digoxigenin probes.
Unfortunately it seems as if the anti-DIG-POD by Roche doesn't work as 
well as the anti-DIG-AP I'm normally using.
Basically, I get a weaker signal with anti-DIG-POD + tyramide 
amplification than with unamplified anti-DIG-AP.

I have also tried biotinylated probes to avoid using anti-DIG-POD 
altogether, but so far I haven't got any signal at all with them.

Any ideas, anyone?

With best regards,
Mikael Niku


  Mikael Niku             URL:
  University of Helsinki  Dept. Basic Veterinary Sciences

  - Mitäkö mieltä olen länsimaisesta sivistyksestä?
  Minusta se olisi erinomainen ajatus!

                                          - Gandhi


Histonet mailing list

This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender.

Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead

This note also confirms that this email message has been
virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses.

Histonet mailing list

<< Previous Message | Next Message >>