[Histonet] non-specific staining IHC

From:"Mrosla, Tina M"

Our lab has recently noticed non-specific staining on our negative antibodies (mouse and rabbit) since we changed our protocols to comply with CAP guidelines. We apply our harshest pre-treatments to our negative controls, including cell conditioner, protease, blocker, and amplification kits. We have a Ventana BenchMark and use their products. I have never experienced background staining before, so I need some advice. Is this a common problem and can it be fixed?
Thank you!

Tina Mrosla
St. Joseph's Hospital 
St. Paul, MN

The information included in this e-mail message, including any attachments, is intended only for the person or organization to which it is addressed. This e-mail message may contain information that is privileged or confidential. If you receive this e-mail message and are not the intended recipient or responsible for delivering the message to the intended recipient, you may not use, disseminate, distribute or copy the information included in this e-mail and any attachments. If you received this e-mail message by mistake, please reply by e-mail and destroy all copies of this message and any attachments. Thank you.

Histonet mailing list

<< Previous Message | Next Message >>