[Histonet] negative controls with IHC
I have a question regarding negative controls for a manual immunoperoxidase procedure. Do you use a neg control for each antibody used with each block? or one neg control per block regardless of how many antibodies for that block. The latter is what I was told, but the first seems more likely on account of each antibody may be treated differently with some needing antigen retrieval--obviously the contol should receive identical treatment. What is the usual neg control protocol?
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