[Histonet] Re: help needed with sheep spine

From:caron fournier

Hi All:
  being new to hard tissue processing I would like to ask for some help with a problem....I have some sheep spines that need to be embedded for sectioning and grinding but they have a pmma implant in them and I'm not sure how to process them without causing some problem with the existing PMMA and still be able to get good results on the cancellous bone in the vertebrae. Any suggestions are welcome.
  Caron

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Today's Topics:

1. software for SOP (Muhammad Tahseen)
2. Service Contracts on Finesse ME Microtomes (Parker, Helayne)
3. Mar-Med Bone Saws (Extenders) (Parker, Helayne)
4. antibodies of bone marker (pex)
5. RE: FISH for HER2 testing (Sebree Linda A.)
6. RE: Anti-DIG peroxidase problem (C.M. van der Loos)
7. RE: FISH for HER2 testing (Temple Nancy)
8. NBF fixed tissue and subsequent first step for dehydration.
(Bruce Abaloz)
9. Mart 1 (karen johnson)
10. defatting (Sandifort, O.M. (PATH))
11. RE: Histonet Digest, Vol 25, Issue 8 (Rice, Michael)
12. RE: FISH for Her-2 testing (Mrosla, Tina M)
13. RE: Anti-DIG peroxidase problem (Tony Henwood)
14. CD133 on Paraffin (Hofecker, Jennifer L)
15. control issue (Mildred Fail)
16. RE: FISH for HER2 testing (Anne Van Binsbergen)
17. RE: RE: Histonet Digest, Vol 25, Issue 5 (Lee & Peggy Wenk)


----------------------------------------------------------------------

Message: 1
Date: Tue, 6 Dec 2005 23:24:19 +1300
From: "Muhammad Tahseen" 
Subject: [Histonet] software for SOP
To: 
Message-ID: <009101c5fa4f$3924ca80$972bfea9@m7c0y4>
Content-Type: text/plain; charset="iso-8859-1"

Hi All,
I am looking software for my SOP, which cover the CAP requirement as per ANP.02888 NOTE 4.
Thanks,

Muhammad Tahseen,
MLT(Punjab Medical Faculty)
MT(JIMTEF)Japan
Histology Supervisor
SKMCH & RC
Lahore Pakistan.


------------------------------

Message: 2
Date: Tue, 6 Dec 2005 12:27:23 -0600
From: "Parker, Helayne" 
Subject: [Histonet] Service Contracts on Finesse ME Microtomes
To: 
Message-ID:
<2FABC6145388F24EBA8CBD6EC165BCCB02AFB9D3@mail1-schc.skaggs.net>
Content-Type: text/plain; charset="us-ascii"

Hello,
I was wondering if anyone could tell me if they have or have not
purchased service contracts (or extended warranties) on the Finesse ME
Microtomes ? I have been asked to weigh out whether it would be more
cost effective off just paying per visit and repair or having the
"plans". Our units have been here just under a year's time. I am new
here and do not know much about the history of them- except that one was
a demo and another brand new and one has had a defective knife holder
replaced. Any input would be greatly appreciated.

Helayne Parker
Skaggs Community Health Center
Branson, Missouri



------------------------------

Message: 3
Date: Tue, 6 Dec 2005 12:34:08 -0600
From: "Parker, Helayne" 
Subject: [Histonet] Mar-Med Bone Saws (Extenders)
To: 
Message-ID:
<2FABC6145388F24EBA8CBD6EC165BCCB02AFB9D4@mail1-schc.skaggs.net>
Content-Type: text/plain; charset="us-ascii"

Hello again,
I saw in the achieves that there is an "extender" that can be
purchased for the Mar-Med saw. The drawback to the saw is that is only
had a 3 inch clearance. With the extender how much more clearance is
offered ? Again thank you for your input in advance.

Sincerely,
Helayne Parker, HT (A.S.C.P.)
Histology Section Head
Skaggs Community Health Center
Branson, Missouri




------------------------------

Message: 4
Date: Wed, 7 Dec 2005 02:44:00 +0800 (CST)
From: pex 

Subject: [Histonet] antibodies of bone marker
To: Histonet@lists.utsouthwestern.edu
Message-ID: <20051206184400.34928.qmail@web15509.mail.cnb.yahoo.com>
Content-Type: text/plain; charset=gb2312

Dear all,

I would like to do immunostaing in mouse bone paraffin-embedded sections or mouse primary osteoblasts, so firstly I need some good antibodies of bone marker( such as alkaline phosphatase, osteocalcin, osteopontin, bone sialoprotein). But I have no ideas about them . If you have much more experience about them in immunostaining, can you do me a favor? Tell me some information about antibodies( company, catalogue number, or lab, etc)
Thanks a lot

Guofeng


---------------------------------
Żᣬ­ͼȴأٶȣϿע 

------------------------------

Message: 5
Date: Tue, 6 Dec 2005 12:58:06 -0600
From: "Sebree Linda A." 
Subject: RE: [Histonet] FISH for HER2 testing
To: "Dawson, Glen" 
Cc: Histonet 
Message-ID:

Content-Type: text/plain; charset="us-ascii"

Hey Glen, that's way too long. We send our slides to the State Lab of
Hygiene in Madison and they do FISH Her/2-neu at least twice per week as
far as I know. Phone number: (608)265-4604. A little farther afield,
you might also check into US Labs, (800)710-1800. We haven't used them
for FISH but we outsource IHC to them and get excellent TAT and quality.

Good luck,

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Clinical & Research Laboratory
DM223-VA
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson,
Glen
Sent: Tuesday, December 06, 2005 11:04 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] FISH for HER2 testing


All,

I have a pathologist who is looking for good turn around time on FISH
HER2 testing. Their current turn around time is 3 weeks and the
clinicians are clamoring for something better. If anyone knows of a
reference lab that can do good FISH HER2 testing in a timely fashion,
please let me know.

On a side note, has anyone experienced any problems with the Dako FDA
approved HercepTest kit when using it on breast core bx's? My Docs are
not confident using this kit (especially on our breast cores). Has
anyone run side by side comparisons between this kit and FISH HER2
testing?

If someone can suggest a good FISH HER2 reference lab, the second set of
questions are moot.

Thank-you In Advance,

Glen Dawson BS, HT & QIHC (ASCP)
IHC Manager
Milwaukee, WI

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------------------------------

Message: 6
Date: Tue, 06 Dec 2005 20:15:01 +0100
From: "C.M. van der Loos" 
Subject: [Histonet] RE: Anti-DIG peroxidase problem
To: histonet@lists.utsouthwestern.edu
Cc: mikael.niku@helsinki.fi
Message-ID: <19df38c19ddd3a.19ddd3a19df38c@amc.uva.nl>
Content-Type: text/plain; charset="us-ascii"


Dear Mikael,

Reading your message I have two remarks that might explain your
strange findings:
1. The used chromogens are of stong influence to the final
sensitivity/efficiency of a detection system. In your case I guess
that with anti-DIG/AP, NBT/BCIP was used which is one of the most
sensitive/efficient chromogens available. Far better
than most peroxidase chromogens. Your remark you wasn't
successful detecting bio-probes with peroxidase points a bit into
this direction. Have you tried for example TrueBlue or Vector
NovaRed as peroxidase chromogens. Both are very
sensitive/efficient!
2. Obviously you are not an enthusiastic kit-user (just like me...).
What did you do in the tyramide amplification step? Especially the
peroxide concentration should be very very low here, otherwise you
will get and adverse effect and certainly no amplification!

Hope this helps.

Chris van der Loos, PhD
Dept. of Pathology
Academic Medical Center M2-230
Meibergdreef 9
NL-1105 AZ Amsterdam
The Netherlands


Date: Tue, 06 Dec 2005 09:08:59 +0200
From: Mikael Niku 
Subject: [Histonet] Anti-DIG peroxidase problem
To: histonet@lists.utsouthwestern.edu
Dear Histonetters,
I'm trying to set up tyramide amplification for my in situ
hybridizations with digoxigenin probes.
Unfortunately it seems as if the anti-DIG-POD by Roche doesn't work as
well as the anti-DIG-AP I'm normally using.
Basically, I get a weaker signal with anti-DIG-POD + tyramide
amplification than with unamplified anti-DIG-AP.
I have also tried biotinylated probes to avoid using anti-DIG-POD
altogether, but so far I haven't got any signal at all with them.
Any ideas, anyone?


------------------------------

Message: 7
Date: Tue, 6 Dec 2005 14:22:07 -0500
From: "Temple Nancy" 
Subject: RE: [Histonet] FISH for HER2 testing
To: 
Message-ID:

Content-Type: text/plain; charset="iso-8859-1"

We send our FISH HER2 to CAD (Center for Advanced Diagnostics, an AmeriPath Lab)in Orlando, Fla. Our turnaround time is about 1 week.

Nancy Temple
Histology
St. Francis Hospital
Indianapolis, Indiana

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Dawson,
Glen
Sent: Tuesday, December 06, 2005 12:04 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] FISH for HER2 testing


All,

I have a pathologist who is looking for good turn around time on FISH HER2
testing. Their current turn around time is 3 weeks and the clinicians are
clamoring for something better. If anyone knows of a reference lab that can
do good FISH HER2 testing in a timely fashion, please let me know.

On a side note, has anyone experienced any problems with the Dako FDA
approved HercepTest kit when using it on breast core bx's? My Docs are not
confident using this kit (especially on our breast cores). Has anyone run
side by side comparisons between this kit and FISH HER2 testing?

If someone can suggest a good FISH HER2 reference lab, the second set of
questions are moot.

Thank-you In Advance,

Glen Dawson BS, HT & QIHC (ASCP)
IHC Manager
Milwaukee, WI

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------------------------------

Message: 8
Date: Wed, 30 Nov 2005 12:15:35 +1100
From: Bruce Abaloz 

Subject: [Histonet] NBF fixed tissue and subsequent first step for
dehydration.
To: Lorraine Rolston 
Message-ID: 

Content-Type: text/plain; charset=us-ascii; format=flowed

Lorraine,

In our Lab. we fix in 70% ETOH without any detrimental effects to 
morphology & then start processing in this also.....am happy to help 
in any way. Cheers,
Bruce in OZ
-- 
BRUCE ABALOZ 
PH:61383446282
HISTOLOGIST 
FAX:61383447909
DEPT.of ZOOLOGY 
EMAIL: brucea@unimelb.edu.au
THE UNIVERSITY Of MELBOURNE. VICTORIA. AUSTRALIA 3010
Nobody Can Make You Feel Inferior Without YOUR Permission - 
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astronaut. 
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------------------------------

Message: 9
Date: Wed, 30 Nov 2005 17:16:50 -0800 (PST)
From: karen johnson 
Subject: [Histonet] Mart 1
To: histonet@lists.utsouthwestern.edu
Message-ID: <20051201011650.86833.qmail@web52305.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

I work in a small dermatology lab and want to start to
do MART-1 on frozen sections especially for lentigo
maligna. I need a procedure that is easy, and pretty
quick. Any help would be appreciated.
THANKS



__________________________________ 
Start your day with Yahoo! - Make it your home page! 
http://www.yahoo.com/r/hs



------------------------------

Message: 10
Date: Fri, 2 Dec 2005 14:23:29 +0100
From: "Sandifort, O.M. \(PATH\)" 
Subject: [Histonet] defatting
To: 
Message-ID:
<40964D91251A18469CB08B6E96FB30BA226257@mailc.lumcnet.prod.intern>
Content-Type: text/plain; charset="iso-8859-1"

Hello,

I'm looking for a method to defat breast tissue and while searching the web I found you question on the internet and now I'm wondering if somebody has answered you of if you found the answer to your question.

Regards

Orchid Sandifort, pathology, labworker Hospital Leiden, the Netherlands (LUMC)


Defatting
<< Previous Message | Next Message >> 
From: "Badley, Carmen M SrA LRMC" 
To: "'histonet@pathology.swmed.edu'" 
Reply-To: 
Date: Thu, 16 Sep 1999 15:31:29 +0200 
Content-Type: text/plain; charset="iso-8859-1" 

I was wondering if anyone has a good procedure on defatting breast tissue?
Thank you

Carmen Badley-Histology
Landstuhl Regional Medical Center, GE





------------------------------

Message: 11
Date: Tue, 6 Dec 2005 14:49:34 -0500
From: "Rice, Michael" 
Subject: [Histonet] RE: Histonet Digest, Vol 25, Issue 8
To: 
Message-ID:
<3BC92F29BE821745AB15E04C98EE028D6D38DB@HCH2KMAIL.holy-cross.com>
Content-Type: text/plain; charset="utf-8"

Hi Glen, We are using USLABS and getting results back in 4-5 days. We also use them for flow and have results back in 24-48 hours. They are great to work with and no, I do not having any financial interest in them
Mike rice
Holy cross hospital
Ft lauderdale

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu
Sent: Tuesday, December 06, 2005 1:12 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 25, Issue 8

Send Histonet mailing list submissions to
histonet@lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
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You can reach the person managing the list at
histonet-owner@lists.utsouthwestern.edu

When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."


Today's Topics:

1. Looking for Dr. Valerie Ray (pathrm35@adelphia.net)
2. RE: active Caspase-3 antibody and TUNEL kit (Melissa Gonzalez)
3. re:MSH-2 and MLH-1 (Emma JONES)
4. specific Ig detection (JEFF TATUM ZELIADT)
5. Re: specific Ig detection (Gayle Callis)
6. Anti-DIG peroxidase problem (Mikael Niku)
7. RE: Histonet Digest, Vol 25, Issue 5 (Gus Mondragon)
8. Bone EM Question (Sheffield, Tiffany L)
9. FISH for HER2 testing (Dawson, Glen)
10. Tropheryma whippleii (ole)
11. Re: FISH for HER2 testing (Rene J Buesa)


----------------------------------------------------------------------

Message: 1
Date: Mon, 5 Dec 2005 13:32:14 -0500
From: 

Subject: [Histonet] Looking for Dr. Valerie Ray
To: histonet@lists.utsouthwestern.edu
Message-ID:
<5120670.1133807534399.JavaMail.root@web6.mail.adelphia.net>
Content-Type: text/plain; charset=utf-8

I am trying to contact Dr. Valerie Anne Yantsos Ray from the Tampa Bay, Florida area. If anyone knows her, please forward this email to her as I would like to speak with her.
Thanks in advance.
Ron Martin
561-721-2400



------------------------------

Message: 2
Date: Mon, 5 Dec 2005 10:46:33 -0800
From: "Melissa Gonzalez" 
Subject: [Histonet] RE: active Caspase-3 antibody and TUNEL kit
To: 
Cc: tenny jin 
Message-ID:

Content-Type: text/plain; charset="us-ascii"

Tenny, 
Try the TUNEL kit from Chemicon, and active Caspase3 from R&D Systems
(not sure if it will x-react with g.p). I used to use the Roche TUNEL
kit, but have not been happy with the consistency of the kit in the past
few years.
Good luck, 
Melissa

-----Original Message-----


Dear Histonetter,
I would like hear some advice on good antibody against active Caspase-3.
Prefer it is working in different species.
Also, could anyone here recommend an apoptosis dectection kit (TUNEL
assay)?
We used to try the one from PerkinElmer.
I am working with guinea pig tissue.
Any input is greatly appreciated!

/Tenny
KI,stockholm






------------------------------

Message: 3
Date: Mon, 5 Dec 2005 20:38:11 +0100
From: "Emma JONES" 
Subject: [Histonet] re:MSH-2 and MLH-1
To: 
Message-ID:


Content-Type: text/plain; charset="windows-1250"



Emma Jones




Hi Diana, 
Have you asked your Ventana rep, for info on these antibodies.
They now sell both these primaries, pre-diluted and with recommended protocol.
MSH 2 Cat # 760-4265
MLH 1 Cat # 760-4264 

Regards
Emma

Message: 12
Date: Fri, 2 Dec 2005 09:49:11 -0500
From: "Goodwin, Diana" 
Subject: [Histonet] MSH-2 and MLH-1
To: "Histonet \(E-mail\)" 
Message-ID:
<80CDD9C3FEEAFD4982B114C4A6DFD00E256F77@uphsmbx2.UPHS.PENNHEALTH.PRV>
Content-Type: text/plain; charset="iso-8859-1"

Greetings, Histonet.

I anyone running paraffin IHC with these Abs for human colo-rectal ca successfully on the Ventana Benchmark, and if so, which clone, vendor, dilution, etc.

Thanks in advance,

Diana Goodwin
Supervisor, Anatomic Pathology
Pennsylvania Hospital, Preston 655-C

ph: 215-829-6532
fax: 215-829-7564
e-mail: goodwind@pahosp.com





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------------------------------

Message: 4
Date: Mon, 05 Dec 2005 21:27:23 +0000
From: "JEFF TATUM ZELIADT" 
Subject: [Histonet] specific Ig detection
To: histonet@lists.utsouthwestern.edu
Message-ID: 
Content-Type: text/plain; format=flowed

I am a student taking an immunobiology course. I was wondering if someone 
could help point me in the right direction. I have an unknown infections 
agent, I know that it produces anaphlylaxis and will produce IgE and IgA. 
If I isolate the IgE's from the serum I will get all IgE's even those not 
specific to the bug. How can I seperate the two and get the specific 

=== message truncated ===  



Caron Fournier, BSc, R.T.
Department of Orthopaedics,
Division of Orthopaedic Engineering Research,
U.B.C.
 

		
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