Very interesting Chris! I have found sometimes this works and 
sometimes it doesn't and you get complete loss of staining - 
frustrating when one was just trying to save time to begin with!! I 
imagine it's antigen dependent but I have not had time to check.

In my opinion Betsy, if you can avoid it (because you are just doing 
it to save time instead of setting up a workshop) then avoid it ... 
it simply isn't worth the risk IMHO.



At 9:52 AM +0100 12/2/05, C.M. van der Loos wrote:
>    Betsy,
>
>    For my NSH wet-workshops I tested the option of going up to endogenous
>    PO blocking, HIER and then overnight storage at 4C in PBS with 1% BSA.
>    Next  day  we  started at the protein blocking step and then the rest.
>    Staining was as good as running the whole procedure in one day.
>
>    Hope this info helps.
>
>    Chris van der Loos, PhD
>    Dept. of Pathology
>    Academic Medical Center M2-230
>    Meibergdreef 9
>    NL-1105 AZ Amsterdam
>    The Netherlands

-- 

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet