[Histonet] RE: Histonet Digest, Vol 25, Issue 8

From:"Rice, Michael"

Hi Glen, We are using USLABS and getting results back in 4-5 days. We also use them for flow and have results back in 24-48 hours. They are great to work with and no, I do not having any financial interest in themMike riceHoly cross hospitalFt lauderdale-----Original Message-----From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.eduSent: Tuesday, December 06, 2005 1:12 PMTo: histonet@lists.utsouthwestern.eduSubject: Histonet Digest, Vol 25, Issue 8Send Histonet mailing list submissions to	histonet@lists.utsouthwestern.eduTo subscribe or unsubscribe via the World Wide Web, visit	http://lists.utsouthwestern.edu/mailman/listinfo/histonetor, via email, send a message with subject or body 'help' to	histonet-request@lists.utsouthwestern.eduYou can reach the person managing the list at	histonet-owner@lists.utsouthwestern.eduWhen replying, please edit your Subject line so it is more specificthan "Re: Contents of Histonet digest..."Today's Topics:   1. Looking for Dr. Valerie Ray (pathrm35@adelphia.net)   2. RE: active Caspase-3 antibody and TUNEL kit (Melissa Gonzalez)   3. re:MSH-2 and MLH-1 (Emma JONES)   4. specific Ig detection (JEFF TATUM ZELIADT)   5. Re: specific Ig detection (Gayle Callis)   6. Anti-DIG peroxidase problem (Mikael Niku)   7. RE: Histonet Digest, Vol 25, Issue 5 (Gus Mondragon)   8. Bone EM Question (Sheffield, Tiffany L)   9. FISH for HER2 testing (Dawson, Glen)  10. Tropheryma whippleii (ole)  11. Re: FISH for HER2 testing (Rene J Buesa)----------------------------------------------------------------------Message: 1Date: Mon, 5 Dec 2005 13:32:14 -0500From: Subject: [Histonet] Looking for Dr. Valerie RayTo: histonet@lists.utsouthwestern.eduMessage-ID:	<5120670.1133807534399.JavaMail.root@web6.mail.adelphia.net>Content-Type: text/plain; charset=utf-8I am trying to contact Dr. Valerie Anne Yantsos Ray from the Tampa Bay, Florida area. If anyone knows her, please forward this email to her as I would like to speak with her.Thanks in advance.Ron Martin561-721-2400------------------------------Message: 2Date: Mon, 5 Dec 2005 10:46:33 -0800From: "Melissa Gonzalez" Subject: [Histonet] RE: active Caspase-3 antibody and TUNEL kitTo: Cc: tenny jin Message-ID:	Content-Type: text/plain;	charset="us-ascii"Tenny, Try the TUNEL kit from Chemicon, and active Caspase3 from R&D Systems(not sure if it will x-react with g.p). I used to use the Roche TUNELkit, but have not been happy with the consistency of the kit in the pastfew years.Good luck, Melissa-----Original Message-----Dear Histonetter,I would like hear some advice on good antibody against active Caspase-3.Prefer it is working in different species.Also, could anyone here recommend an apoptosis dectection kit (TUNELassay)?We used to try the one from PerkinElmer.I am working with guinea pig tissue.Any input is greatly appreciated!/TennyKI,stockholm------------------------------Message: 3Date: Mon, 5 Dec 2005 20:38:11 +0100From: "Emma JONES" Subject: [Histonet] re:MSH-2 and MLH-1To: Message-ID:		Content-Type: text/plain;	charset="windows-1250"Emma Jones     Hi Diana, Have you asked your Ventana rep, for info on these antibodies.They now sell both these primaries, pre-diluted and with recommended protocol.MSH 2 Cat # 760-4265MLH 1 Cat # 760-4264 RegardsEmmaMessage: 12Date: Fri, 2 Dec 2005 09:49:11 -0500From: "Goodwin, Diana" Subject: [Histonet] MSH-2 and MLH-1To: "Histonet \(E-mail\)" Message-ID:	<80CDD9C3FEEAFD4982B114C4A6DFD00E256F77@uphsmbx2.UPHS.PENNHEALTH.PRV>Content-Type: text/plain;	charset="iso-8859-1"Greetings, Histonet. I anyone running paraffin IHC with these Abs for human colo-rectal ca successfully on the Ventana Benchmark, and if so, which clone, vendor, dilution, etc. Thanks in advance, Diana GoodwinSupervisor, Anatomic PathologyPennsylvania Hospital, Preston 655-C ph:  215-829-6532fax:  215-829-7564e-mail:  goodwind@pahosp.com -- No virus found in this outgoing message.Checked by AVG Free Edition.Version: 7.1.362 / Virus Database: 267.13.11/191 - Release Date: 02/12/2005 ------------------------------Message: 4Date: Mon, 05 Dec 2005 21:27:23 +0000From: "JEFF TATUM ZELIADT" Subject: [Histonet] specific Ig detectionTo: histonet@lists.utsouthwestern.eduMessage-ID: Content-Type: text/plain; format=flowedI am a student taking an immunobiology course.  I was wondering if someone could help point me in the right direction.  I have an unknown infections agent, I know that it produces anaphlylaxis and will produce IgE and IgA.  If I isolate the IgE's from the serum I will get all IgE's even those not specific to the bug.  How can I seperate the two and get the specific antigen?  Can I use a protein which would be specific to the bug but how do I know what protein?------------------------------Message: 5Date: Mon, 05 Dec 2005 15:02:18 -0700From: Gayle Callis Subject: Re: [Histonet] specific Ig detectionTo: "JEFF TATUM ZELIADT" ,	Histonet@lists.utsouthwestern.eduMessage-ID:	<6.0.0.22.1.20051205144633.01b2e820@gemini.msu.montana.edu>Content-Type: text/plain; charset="us-ascii"; format=flowedYou say "I know that it produces anaphlylaxis and will produce IgE and IgA so isn't this called the Host response.  In other words the host infected by the "bug" responds to this by producing IgE (an allergic response as is anaphylaxis  along with IgA)  I don't think the bug is producing these Ig's.I suggest you try to get your "bug" identified via some microbiological means, such as a diagnostic culturing protocol.The serum of an animal infected with this infectious agent should contain antiserum to the agent, hence antiserum i.e. antibodies to the bug should be present in the serum of the infected host.   So if you infected mouse cells with the bug, then the serum of the mouse will contain mouse antiBug antibodies - not necessarily IgA.I guess I am confused on what you are trying to do here.  At 02:27 PM 12/5/2005, you wrote:>I am a student taking an immunobiology course.  I was wondering if someone >could help point me in the right direction.  I have an unknown infections >agent,>If I isolate the IgE's from the serum I will get all IgE's even those not >specific to the bug.  How can I seperate the two and get the specific >antigen?  Can I use a protein which would be specific to the bug but how >do I know what protein?>>>>_______________________________________________>Histonet mailing list>Histonet@lists.utsouthwestern.edu>http://lists.utsouthwestern.edu/mailman/listinfo/histonetGayle CallisResearch Histopathology SupervisorVeterinary Molecular BiologyMontana State University - BozemanPO Box 173610Bozeman MT 59717-3610406 994-6367406 994-4303 (FAX)------------------------------Message: 6Date: Tue, 06 Dec 2005 09:08:59 +0200From: Mikael Niku Subject: [Histonet] Anti-DIG peroxidase problemTo: histonet@lists.utsouthwestern.eduMessage-ID: <4395390B.8040003@helsinki.fi>Content-Type: text/plain; charset=ISO-8859-1; format=flowedDear Histonetters,I'm trying to set up tyramide amplification for my in situ hybridizations with digoxigenin probes.Unfortunately it seems as if the anti-DIG-POD by Roche doesn't work as well as the anti-DIG-AP I'm normally using.Basically, I get a weaker signal with anti-DIG-POD + tyramide amplification than with unamplified anti-DIG-AP.I have also tried biotinylated probes to avoid using anti-DIG-POD altogether, but so far I haven't got any signal at all with them.Any ideas, anyone?With best regards,Mikael Niku-- ////////////////////////////////////////////////////////////  Mikael Niku             URL: www.helsinki.fi/~mniku/  University of Helsinki  Dept. Basic Veterinary Sciences  - Mitäkö mieltä olen länsimaisesta sivistyksestä?  Minusta se olisi erinomainen ajatus!                                          - Gandhi////////////////////////////////////////////////////////////------------------------------Message: 7Date: Tue, 6 Dec 2005 03:29:46 -0500From: "Gus Mondragon" Subject: [Histonet] RE: Histonet Digest, Vol 25, Issue 5To: Message-ID:	<03B63DE44B5C014D84F9A9D8A968B5174C4D3E@lithium.corp.gsopath.com>Content-Type: text/plain;	charset="iso-8859-1"A positive and a negative control is required by CLIA in all AFB stains (Leprosy and TB). A known positive control validates your staining procedure; a negative control eliminates the presence of artifact in your sections Gus Mondragon gmondragon@gsopath.com-----Original Message-----From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.eduSent: Saturday, December 03, 2005 1:09 PMTo: histonet@lists.utsouthwestern.eduSubject: Histonet Digest, Vol 25, Issue 5Send Histonet mailing list submissions to	histonet@lists.utsouthwestern.eduTo subscribe or unsubscribe via the World Wide Web, visit	http://lists.utsouthwestern.edu/mailman/listinfo/histonetor, via email, send a message with subject or body 'help' to	histonet-request@lists.utsouthwestern.eduYou can reach the person managing the list at	histonet-owner@lists.utsouthwestern.eduWhen replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..."Today's Topics:   1. Re: RE: Paraffin sections (Maria Mejia)   2. Extracellular matrix proteins fixation. Details      (Diego J. Rodr?guez Gil)   3. RE: Specail stains Negative controls (Smith, Jeffery D.  (HSC))   4. Re: Specail stains Negative controls (Bryan Hewlett)   5. RE: Goat polymer (pruegg@ihctech.net)   6. Knife holder (arunams)   7. RE: Question: Inventory (Favara, Cynthia (NIH/NIAID))   8. AW: [Histonet] Question: Inventory (Gudrun Lang)----------------------------------------------------------------------Message: 1Date: Fri, 02 Dec 2005 10:16:05 -0800From: Maria Mejia Subject: Re: [Histonet] RE: Paraffin sectionsTo: "Andrea T. Hooper" Cc: histonet@lists.utsouthwestern.edu,	"C.M. van der Loos"	,	BMolinari@heart.thi.tmc.eduMessage-ID: <43908F65.6010808@ski.org>Content-Type: text/plain; charset=us-ascii; format=flowedThis type of experimenting would be interesting to check out especially withlower percentages of BSA. Andrea, I would agree that this overnight storage step probably is antigen dependent (Chris, that's what I meant by too much) and it would require a whole gamut testing of antibodies to see which respondpositively and which would not...if one is inclined to do so.YoursMaria Bartola MejiaSmith-Kettlewell Eye Research InstituteSan Francisco, CA 94115Email: maria@ski.orgPhone: (415)-345-2185Andrea T. Hooper wrote:> Very interesting Chris! I have found sometimes this works and> sometimes it doesn't and you get complete loss of staining - > frustrating when one was just trying to save time to begin with!! I > imagine it's antigen dependent but I have not had time to check.>> In my opinion Betsy, if you can avoid it (because you are just doing> it to save time instead of setting up a workshop) then avoid it ... it > simply isn't worth the risk IMHO.>>>> At 9:52 AM +0100 12/2/05, C.M. van der Loos wrote:>>>    Betsy,>>>>    For my NSH wet-workshops I tested the option of going up to endogenous>>    PO blocking, HIER and then overnight storage at 4C in PBS with 1% BSA.>>    Next  day  we  started at the protein blocking step and then the rest.>>    Staining was as good as running the whole procedure in one day.>>>>    Hope this info helps.>>>>    Chris van der Loos, PhD>>    Dept. of Pathology>>    Academic Medical Center M2-230>>    Meibergdreef 9>>    NL-1105 AZ Amsterdam>>    The Netherlands>>------------------------------Message: 2Date: Fri, 02 Dec 2005 13:55:04 -0500From: "Diego J. Rodr?guez Gil"	Subject: [Histonet] Extracellular matrix proteins fixation. DetailsTo: histonet@lists.utsouthwestern.eduMessage-ID: <1133549704.43909888f401e@webmail.med.yale.edu>Content-Type: text/plain; charset=ISO-8859-1Hi:     Thanks a lot to all of you who answer!. Since some of you asked, I am sending some more details.     I am working on mice (CD-1), and the molecules I am trying to stain are named Wnt (Wingless-Int). Antibodies are from R&D systems (most of them are goats), and there are no previous works reporting the use of these antibodies. I am only making the assumption that they work, based on the datasheet the company provides.  I could see some very faint positive stain for some of them, but I am sure I losing some because of fixation. Besides that, I know they are expressed based on RT-PCR and in situ hybridization experiments.     Brief protocol is:         Perfusion with 4% PFA, and then overnight deep in 4% PFA at 4 C. Transfer to PBS, then 30% sucrose til they sink, freeze on OCT and then section on cryostat at -18C.         The antigen retrieval protocols that I tried were steaming (2, 5 and 10 min with sodium citrate) or pepsin in HCl. None of them gave me any better stainings.         Staining protocol: Wash in TBS and then add 2% BSA in TBS-T, for 30 min. Primary antibody (on 2% BSA) Over night .	Wash 3 x TBS-T.	Add secondary antibody on 2% BSA for an hour.	Wash 2 x TBS-T.	Wash TBS and mount with Crystal Mount.      Thanks again!      Best regards,      Diego.-- Diego J. Rodríguez GilPostdoctoral AssociateDepartment of NeurosurgeryYale University School of MedicinePO Box 208082333 Cedar St, FMB-430New Haven CT, 06520-8082U.S.A.------------------------------Message: 3Date: Fri, 2 Dec 2005 13:23:19 -0600From: "Smith, Jeffery D.  \(HSC\)" Subject: RE: [Histonet] Specail stains Negative controlsTo: "Grant, Debra, R" ,	Message-ID:	Content-Type: text/plain;	charset="iso-8859-1" Debby,    Routine special stains only require a positive control as you are looking to see if the stain worked or not.  Specimen is either positive or negative.  No need for negative control.  IHC uses both pos. and neg. controls because you are looking for varying intensities of staining as well as pos. or neg. Best of Luck, Jeff________________________________Hi Histonetters,Does anyone run negative control slides with their GMS, AFB, and Gram special stains, and if so why?Kind Regards,Debby R. Grant HT(ASCP)Histology CoordinatorThe Children's Mercy Hospital & Clinics2401 Gillham Rd.Kansas City, Missouri 64108lab (816)234-3827fax (816) 802-1492_______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet------------------------------Message: 4Date: Fri, 2 Dec 2005 15:23:28 -0500From: "Bryan Hewlett" Subject: Re: [Histonet] Specail stains Negative controlsTo: "Smith, Jeffery D.  (HSC)" ,	"Grant,	Debra, R" , Message-ID: <002001c5f77e$786f0990$6400a8c0@mainbox>Content-Type: text/plain; format=flowed; charset="iso-8859-1";	reply-type=originalDebby and Jeff,Negative control slides are useful for GMS, Gram and AFB in order to eliminate false positives due to contamination from the reagents, washing, carry-over etc.Regards,Bryan----- Original Message ----- From: "Smith, Jeffery D. (HSC)" To: "Grant, Debra, R" ; Sent: Friday, December 02, 2005 2:23 PMSubject: RE: [Histonet] Specail stains Negative controlsDebby,   Routine special stains only require a positive control as you are looking to see if the stain worked or not.  Specimen is either positive or negative. No need for negative control.  IHC uses both pos. and neg. controls because you are looking for varying intensities of staining as well as pos. or neg.Best of Luck, Jeff________________________________Hi Histonetters,Does anyone run negative control slides with their GMS, AFB, and Gram special stains, and if so why?Kind Regards,Debby R. Grant HT(ASCP)Histology CoordinatorThe Children's Mercy Hospital & Clinics2401 Gillham Rd.Kansas City, Missouri 64108lab (816)234-3827fax (816) 802-1492_______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet_______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet------------------------------Message: 5Date: Fri, 2 Dec 2005 13:39:43 -0700From: pruegg@ihctech.netSubject: RE: [Histonet] Goat polymerTo: "'Orr, Rebecca'" ,	Message-ID: <200512022039.jB2KdkXX005415@pro12.abac.com>Content-Type: text/plain;	charset="us-ascii"Becky,Invitrogen is the company who makes the goat labeled polymer.  As for blocking, I use serum free protein block before the primary and then before the rab. Anti-goat secondary and then again before the rabbit labeled polymer.  If I still encounter non-specific staining I put some serum (from the host of the secondary, about 10%) in with the protein block.  This method is very sensitive and you can bring up lots of non-specific BG so be careful to optimize your dilutions and times for the antibody and for the detection reagents. Patsy-----Original Message-----From: histonet-bounces@lists.utsouthwestern.edu[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Orr, RebeccaSent: Friday, December 02, 2005 6:35 AMTo: histonet@lists.utsouthwestern.eduSubject: [Histonet] Goat polymerHello everyone,Just recently there was a posting on how to run a rabbit polymer with goat antibodies...or something to that nature, using an anti rabbit secondary and then the Dako polymer...could that person send me an email please?  I had a quick question about blocking... Also, does anyone have the name of the company that makes a goat polymer?  I know there's a company out there...I've seen the ads but can't locate it...Many thanks and have a great weekend!Becky  Becky Orr CLA,HT(ASCP)IHC Lead Evanston Northwestern Healthcare847-570-2771 _______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet------------------------------Message: 6Date: Fri, 02 Dec 2005 14:04:38 -0800 (PST)From: arunams Subject: [Histonet] Knife holderTo: histonet@lists.utsouthwestern.eduMessage-ID:	<5641228.1133561078869.JavaMail.myubc2@portal9.itservices.ubc.ca>Content-Type: text/plain; charset=us-asciiHi AllDoes eny one know where to find a Tungston Carbide Knive holder for a Sorvall JB-4 Microtome. I really need one urgently. Thanks for your help Aruna Somasiri------------------------------Message: 7Date: Fri, 2 Dec 2005 17:35:26 -0500From: "Favara, Cynthia \(NIH/NIAID\)" Subject: RE: [Histonet] Question: InventoryTo: "Luis Chiriboga" ,	"Histonet"	Message-ID:	Content-Type: text/plain;	charset="us-ascii"I do not do high volume consistently but have some weeks where I am doing 200-300 stains per week. I do not register my reagents as the come into the lab. cCynthia FavaraNIAID/NIH/RML/LPVD903 South 4th StreetHamilton, MT 59840406-363-9317Disclaimer: The information in this e-mail and any of its attachments is confidential and may contain sensitive information. It should not be used by anyone who is not the original intended recipient. If you have received this e-mail in error please inform the sender and delete it from your mailbox or any other storage devices. National Institute of Allergy and Infectious Diseases shall not accept liability for any statements made that are sender's own and not expressly made on behalf of the NIAID by one of its representatives-----Original Message-----From: Luis Chiriboga [mailto:Luis.Chiriboga@med.nyu.edu] Sent: Thursday, December 01, 2005 11:14 AMTo: HistonetSubject: [Histonet] Question: InventoryHi AllFor those of you who are in high volume labs using ventana stainers, do you register all your reagents when they arrive? or do you register as you use? Thanks in advance Luis _______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet------------------------------Message: 8Date: Sat, 3 Dec 2005 08:52:40 +0100From: "Gudrun Lang" Subject: AW: [Histonet] Question: InventoryTo: "Histonetliste \(Histonetliste\)"	Message-ID:	Content-Type: text/plain;	charset="us-ascii"We register the bulk-reagents and prep-kits in the Ventana System just before use. The antibodies are registered, when they arrive, to have them on the list.Gudrun LangHi AllFor those of you who are in high volume labs using ventana stainers, do you register all your reagents when they arrive? or do you register as you use? Thanks in advance Luis _______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet_______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet------------------------------_______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonetEnd of Histonet Digest, Vol 25, Issue 5***************************************------------------------------Message: 8Date: Tue, 6 Dec 2005 08:11:03 -0600From: "Sheffield, Tiffany L" Subject: [Histonet] Bone EM QuestionTo: Message-ID:	Content-Type: text/plain;	charset="us-ascii"Hi Fellow Histonetters! All you EM pro's, I have a question for you...What has been yourexperience with bone and EM? What is the largest sample size you haveworked with (of course decaled)? I have been under the impression thatonly very small pieces(or very young bone that is not very calcifiedyet) that are very de-caled yield acceptable results. I am hearingconflicting accounts as to sample size and whether it has to be decaledor not. It is my understanding that it does have to be decaled and verysmall if it has a shot of working. I have only worked with soft tissuesamples for EM. Any help would be greatly appreciated. Thanks! Tiffany Sheffield-Lopez, HT(ASCP)Supervisor, Bone Histomorphometry & Biomaterials LabDepartment of Orthopaedic SurgeryUTHSC-Houston Medical School6431 Fannin, Suite 6.144 MSBHouston , TX 77030713-500-6803 WK713-500-0729 Fax ------------------------------Message: 9Date: Tue, 6 Dec 2005 11:03:34 -0600 From: "Dawson, Glen" Subject: [Histonet] FISH for HER2 testingTo: Histonet@lists.utsouthwestern.eduMessage-ID: Content-Type: text/plain;	charset="iso-8859-1"All,I have a pathologist who is looking for good turn around time on FISH HER2testing.  Their current turn around time is 3 weeks and the clinicians areclamoring for something better.  If anyone knows of a reference lab that cando good FISH HER2 testing in a timely fashion, please let me know.On a side note, has anyone experienced any problems with the Dako FDAapproved HercepTest kit when using it on breast core bx's?  My Docs are notconfident using this kit (especially on our breast cores).  Has anyone runside by side comparisons between this kit and FISH HER2 testing?If someone can suggest a good FISH HER2 reference lab, the second set ofquestions are moot.Thank-you In Advance,Glen Dawson  BS, HT & QIHC (ASCP)IHC ManagerMilwaukee, WI------------------------------Message: 10Date: Tue, 6 Dec 2005 18:06:29 +0100From: "ole" Subject: [Histonet] Tropheryma whippleiiTo: Message-ID: <000c01c5fa87$66d214a0$0100000a@ole>Content-Type: text/plain;	charset="iso-8859-1"Anyone know of a commercially available Anti-Tropheryma Wippleii antibody that works on FFPET?Regards Ole J SteffensenLab scientistDep of Pathology Aalesund Norway------------------------------Message: 11Date: Tue, 6 Dec 2005 09:48:57 -0800 (PST)From: Rene J Buesa Subject: Re: [Histonet] FISH for HER2 testingTo: "Dawson, Glen" ,	Histonet@lists.utsouthwestern.eduMessage-ID: <20051206174857.93672.qmail@web61216.mail.yahoo.com>Content-Type: text/plain; charset=iso-8859-1Hi Glen:  I cannot recommend you a reference lab because we used to do our own tests for the DAKO and the FISH (from VYSIS).  The results agreed and we used to run the DAKO tests on Thursdays and the FISH on Fridays on the cases requested from Thursday of one week to Wednesdays of the following.  Rene J."Dawson, Glen"  wrote:  All,I have a pathologist who is looking for good turn around time on FISH HER2testing. Their current turn around time is 3 weeks and the clinicians areclamoring for something better. If anyone knows of a reference lab that cando good FISH HER2 testing in a timely fashion, please let me know.On a side note, has anyone experienced any problems with the Dako FDAapproved HercepTest kit when using it on breast core bx's? My Docs are notconfident using this kit (especially on our breast cores). Has anyone runside by side comparisons between this kit and FISH HER2 testing?If someone can suggest a good FISH HER2 reference lab, the second set ofquestions are moot.Thank-you In Advance,Glen Dawson BS, HT & QIHC (ASCP)IHC ManagerMilwaukee, WI_______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet  		--------------------------------- Yahoo! Personals Single? There's someone we'd like you to meet. Lots of someones, actually. Yahoo! Personals------------------------------_______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonetEnd of Histonet Digest, Vol 25, Issue 8***************************************
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