[Histonet] Paraffin processing of muscle
Sorry, I forgot to put anything in the subject with my first post, so I am
resubmitting it with a subject line!
I have a question for you about muscle processing for paraffin embedding.
Starting from fixation to embedding, can you tell me the best and most
successful way to do this? I have processed leg muscle (gastroc and soleus)
after initial overnight fixation in 3% paraformaldehyde using the following
schedule on our Excelsior:
Alcohols 1 through 6 45 minutes each
Xylenes substitute 1 through 3 1 hour each
1st Paraffin @ 62 degrees 1.5 hours
2nd Paraffin 1.5 hours
3rd Paraffin 2 hours
I know the paraffin sounds long, but we usually process fatty tissue and
brain that seems to require a longer infiltration time.
What do you think, am I missing the mark? Our muscle seems to have a little
more space surrounding the bundles than the investigator had at their old
I really appreciate any help you can give.
Jo Dee Fish
Jo Dee Fish
Research Technologist III
Gladstone Institute of Cardiovascular Disease
Telephone: (415) 734-2567
Fax: (415) 355-0230
The J. David Gladstone Institutes
1650 Owens Street
San Francisco, CA 94158
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