[Histonet] Extracellular matrix proteins fixation.

From:"Diego J. =?iso-8859-1?b?Um9kcu1ndWV6?= Gil"

Hi everybody:

   I am brand new on the list, so I do not know if this question was posted
before. I apologize for this.
   I am trying to characterize a family of secreted proteins in the Nervous
System by immunohistochemistry, and with the regular fixation protocol that we
used (intracardiac perfusion with 4% PFA), I am not getting any good results.
Not even after some antigen-retrieval procedures. Before trying random things,
does anybody have a good protocol to get extracellular proteins well fixed? It
doesn't matter if it is set up for some other tissue, I would be glad to try
other protocol(s) if there are any available.
   Thank you very much for your help,
   Best regards,

Diego J. Rodríguez Gil
Postdoctoral Associate
Department of Neurosurgery
Yale University School of Medicine
PO Box 208082
333 Cedar St, FMB-430
New Haven CT, 06520-8082
E-mail: diego.rodriguezgil@yale.edu

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