I  have  used  both  DAPI  and  Hoechst,  they  =  are  about the same
   excitation/emission (360/470). If you buy the DAPI chemi= cal, you can
   stain  with  it  at the same time as the secondary antibody, and = you
   should  use  it at a very low conc. (about 1:10,000, I think), so your
   st= ock solution will last forever.

   Mel

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