[Histonet] DAPI vs. Hoescht
I would like to stain my sections with something to highlight the
nucleus. I have heard that both DAPI and Hoescht are good. Is there
are reason to use one over the other? I am looking for something
very quick, easy and inexpensive. I know that Molecular Probes makes
an anti-fade with DAPI, but this is rather expensive and I am worried
about not being able to optimize the conditions.
I am mainly interested in hearing info on the best formulation to
use, a reliable company to purchase from, and any simple protocol you
may have on hand.
I am staining liver sections, 10 micron, NBF fixed, cryoprotected.
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