[Histonet] Cuting speed and sem-thin plastic sectioning
From: | Lawrence D Lanberg/O/VCU |
Hello,
I am a beginning microtomist - = I do this in between classes for the
most part - in a Biochemistry laborato= ry. I semi-thin section murine
retinas using plastic (Spurr's Formula).&nbs= p;I use a Sorvall
MT-1000 ultramicrotome. My sectioning, staining and = destaining has
developed very well in the 4 months I have done this. I have= one
little thing, though, that seems to be impeding progress: Speed of
cut= ting.
Currently, the only way I get quality sec= tions is when I cut at 0.3
mm/sec. Which is almost the slowest setting on t= he machine. If I am
lucky, I may get three blocks done per hour; 20 section= s per-two
slides per block. This is slow output, from what I am told.
I have experimented cutting these on a speed as high = as 7.3 mm/sec.
This greatly increases my output, obviously, but after staining-destaining the sections appear corrugated. But they are
beautiful = at 0.3 mm/sec - the terribly slow speed.
I am un= clear as to why the higher speeds I use give this unwanted,
cordurouy-like = appearance after staining. Considering that I use
plastic (hard formulation= to avoid wrinkles) and that I cut with
self-made glass knives, can anybody= provide a tip as to how I can
possibly cut on the higher speeds, while mai= ntaing quality of
section?
Thank You
&= nbsp;
Larry Lanberg
Student Worker IST
Dept. = of Biochemistry
Virginia Commonwealth University
(804) = 828-7974
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