[Histonet] Bone EM Question

From:"Sheffield, Tiffany L"

Hi Fellow Histonetters!
 All you EM pro's, I have a question for you...What has been your
experience with bone and EM? What is the largest sample size you have
worked with (of course decaled)? I have been under the impression that
only very small pieces(or very young bone that is not very calcified
yet) that are very de-caled yield acceptable results. I am hearing
conflicting accounts as to sample size and whether it has to be decaled
or not. It is my understanding that it does have to be decaled and very
small if it has a shot of working. I have only worked with soft tissue
samples for EM. Any help would be greatly appreciated. 
Tiffany Sheffield-Lopez, HT(ASCP)
Supervisor, Bone Histomorphometry & Biomaterials Lab
Department of Orthopaedic Surgery
UTHSC-Houston Medical School
6431 Fannin, Suite 6.144 MSB
Houston , TX 77030
713-500-6803 WK
713-500-0729 Fax
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