It seems most people use enzyme digestion with FFPE CD31 on mouse, rather 
than an antigen retrieval i.e. with buffers/HIER.

Other message:

I just completed a project usind CD31 on FFPE mouse tissue with good results.
The supplier of the antibody is Research Diagnostics (973-584-7093;
www.researchd.com).  I used rat anti-mouse CD31, monoclonal; Catalog#
RDI-mCD31abrt.  They also suggested zinc fixation, but it worked on formalin
fixation.  I used DAKO's Prot K to open up the sights.

Bob Meyer
Pathology Core Facility
Northwestern University
Gayle Callis HTL, HT, MT(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University
Bozeman MT 59717


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