Re: [Histonet] BCIP/NBT with blocking buffer
The answer is theroretcially - yes. However, this is not the endogenous
enzyme you need to block for this chromogen. NBT/BCIP is a chromogen for
alkaline phosphatase method, so blocking endogenous peroxidase is
unnecessary. You need to put levamisole in with chromogen to block
endogenous alkaline phosphatase found in tissues. Instruction sheets
usually tell you this. Levamisole is sold separately i.e. Vector has it.
OR are you doing a double stain? with both HRP and AP methods. If that
is the case, you would still need to block for endog perox for the the HRP
portion of staining AND block with levamisole with AP portion.
At 12:18 PM 12/22/2004, you wrote:
>Hello. Can anyone tell me if I can block endogenous peroxidase with 0.3%
>H2O2 in sodium azide and still visualize with BCIP/NBT on frozen sections?
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Veterinary Molecular Biology
Montana State University - Bozeman
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