RE: [Histonet] storage of cryosections
Once the tissue is well fixed (24 hours in fixative?), storage in PBS or
in Fixative is not critical. Storage in sucrose until it sinks will
ensure that freezing rate is not as critical, since smaller and fewer
crystals will form in the disruptive sucrose environment.
Charles W. Scouten, Ph.D.
5918 Evergreen Blvd.
St. Louis, MO 63134
Ph: 314 522 0300
FAX 314 522 0377
From: Frouwke Kuijpers [mailto:F.Kuijpers@science.ru.nl]
Sent: Thursday, December 16, 2004 8:00 AM
To: Charles Scouten
Subject: Re: [Histonet] storage of cryosections
Charles, a question:
When the tissue is emersed in sucrose 30% is the storage than less
critical because there are hardly any cristals formed?
Dept. Cellular Animal Physiology
University of Nijmegen
6525 ED Nijmegen
Histonet mailing list
<< Previous Message | Next Message >>