Kris

I've had the same kind of problem working with DAB on skin slides, but
then I changed to another chromogen called NovaRed. The color is brick
red and differentiates very well between the NovaRed and the melanine
cells. I ordered this NovaRed from Vector. Hopes this will help you a
bit.

Joost Bruijntjes
TNO Nutrition and Food Research
PO Box 360
3700 AJ Zeist
The Netherlands
 

-----Oorspronkelijk bericht-----
Van: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] Namens Kristopher
Kalleberg
Verzonden: maandag 20 december 2004 19:29
Aan: histonet@lists.utsouthwestern.edu
Onderwerp: [Histonet] catalase

I am currently working with catalase and I need to find a stain that
will not
be picked up in the melanin of melamocytes.  I have used nuclear fast
red and
DAB but the red-brown color is located in both corneum and melanocytes.
I need
to be able to tell where exactly the catalase is alone.  Thank you in
advance.

Kris

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