Hi,
Whilst we use say 12 mins in a bath of Trypsin to digest for CD21  detection it may also be worth noting that at time we worry, our control tonsilar tissue has varying numbers of follicles and varying degrees of "exposure" though a block  (maybe fixation differences).  So observed staining in the control may change as one goes through a block. ( And we use Envision on a DAKO Techmate 500Plus )
That's all that comes to mind, Happy Stress-free Xmas Immuno 2U

Dave Edmondson
Christie Hospital
Manchester UK

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Martha
Ward
Sent: 21 December 2004 15:03
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] CD21


We are having a little problem with our CD21 and I wondered what other
people are doing?  Currently we are using the Dako antibody, a 1:10
dilution, Protease 2 on the NexEs.  I also have a Dako instrument and
Envision Plus reagents.  Any help/tips would be appreciated.
 
Martha Ward
Wake Forest University Baptist Medical Center
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