[Histonet] re CD21
I guess you are using the 1F8 clone form Dako?
In the datasheet CD21 (1F8) its suggested to use (HIER)heat induced epitopal retrieval - TRS buffer pH 6,1 code no s1699 or s1700.
I am using the s 1699 on CD21 and i think its superior to proteases. Staining intensity using proteases on eg CD21 varies much du to different fixation times in eg formaldehyde. Differences in staining intensity due to different fixation time is largly eliminated with the use of HIER and s-1699 or s-1700.
I use the envision + system on this one, dilution CD21=1:30, 60min incubation roomtemp.
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