[Histonet] rabbit bone specimens
I am working on a research projects with rabbit femurs and tibias. They
are decalcified in cal-ex from fisher for 2 weeks and then processed for
1.5 hours in each solution on the processor. 2 changes of 70, 95, 100%,
xylene and then paraffin. I am really having problems with the cartilage
folding right at the curve of the specimen. It seems as though the bone
just below the cartilage is not thoroughly processed or hard. When I do
feel I get a decent section it seems to fold when I stain. I am using plus
slides. I don't normally do a lot of research so if anyone can help me with
this problem either with cutting or processing solutions I would really
appreciate the help.
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