[Histonet] rabbit bone specimens

From:Mary Lloyd

I am working on a research projects with rabbit femurs and tibias.  They 
are decalcified in cal-ex from fisher for 2 weeks and then processed for 
1.5 hours in each solution on the processor.  2 changes of 70, 95, 100%, 
xylene and then paraffin.  I am really having problems with the cartilage 
folding right at the curve of the specimen.  It seems as though the bone 
just below the cartilage is not thoroughly processed or  hard. When I do 
feel I get a decent section it seems to fold when I stain.  I am using plus 
slides. I don't normally do a lot of research so if anyone can help me with 
this problem either with cutting or processing solutions I would really 
appreciate the help.

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