[Histonet] immunohistochemestry on heart tissue
|From:||"Polonskaya, Yelena" |
My question is for those who works with heart tissue.
When I'm doing immunohistochemistry on heart tissue, I have a very high
background. Sometimes, even if I don't use primary, or
any antibody, I still have florescent staining. I
work with perfused tissue ( 4%PFA) 20 microns.
If anyone can offer a possible solution I would
appreciate the help
Thank you very much
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