[Histonet] Testes - Fixation, Staining, Staging
We are in process of standerdising staging of spermatogenesis in Rats.I
have following questions
Which is the best fixative?Bouin's or modified Davidson's (MD)? Recently
an STP recommendation says MD is the best suited.
What is the best fixation duration in Bouins fluid?Do we need to wash the
same in 70% alcohol after fixation in Bouins? If so please let me know for
Does anybody know the composition of MD?
I would like to know the procedure of PAS -H & E staining for testes for
Can somebody tell me a good reference for staging of the spermatogenesis
in Rats? Also aI would like to know a good CD rom available for the same.
Dr. M.R. Balaji
Dept. Pre clinical safety evaluation,
Dr. Reddys Laboratories ltd. Bollaram Road,
Miyapur, Hyderabad, 500 049
Andhra Pradseh, INDIA
Email - firstname.lastname@example.org
Tel: 040- 23045439 - Ext.432.
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