Hi Martha

Although it's been 2 -3 years since I did hands on IHC I hope this maybe of
some help. In my experience CD21 is fixation dependant, the longer the
tissue is fixed the longer you need to give it in enzyme. At the time I was
doing them CD21 did not survive HEIR, although I beleive that option may
now be available.  So my suggestion is to try different times in Protease

Good luck

Best wishes to all for Xmas and a happy New Year

John


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