We are having a problem with background staining on Kappa and Lambda stains.  
We use DAKO rabbit polyclonal antibodies, DAKO TRS for antigen retrieval in 
the steamer, Envision+ HRP, DAB.  The background staining is especially bad on 
Bouin's fixed, decaled bone marrows.  Does anyone else have this problem?   Do 
you get cleaner staining with monoclonal antibodies?  We are open to 
suggestions.

Thanks in advance,

Ellen Yee, HT
Lead Tech, Immuno Dept.
Central Histology Facility
Diagnostic Pathology Medical Group
2420 J St.
Sacramento, CA 95816
ph: 916-447-2718
fax: 916-447-0620
Sacramento, CA 
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